A ChIP assay further supported the hypothesis that the GLI1 protein directly interacted with and bound the −1350/−652 bp CtBP2 promoter fragment. Overexpression of GLI1 increased the CtBP2 promoter occupancy of GLI1 in Huh7 cells (Figure 5E). Therefore, the results of our experiments implied that GLI1 mediates CtBP2 expression by directly binding its promoter in HCC cells.
