The recently reported modified approach DNase I-released fragment-length analysis of hypersensitivity (DNase-FLASH) [150] allows simultaneous probing of TF occupancy, interactions between TFs and nucleosomes and nucleosome occupancy at individual loci, similar to ATAC-seq. The method is based on the concurrent quantitative analysis of different size fragments released from DNase I digestion of genomic DNA, with microfragments (<125 bp) depicting TF occupancy, and larger fragments (126 to 185 bp) representative of nucleosomal elements.
