In the present study, we provide a framework for the differentiation process of hiPSC-derived NPCs. Compare to some researchers19, our neural differentiation model has higher effectiveness in converting iPSC into neuron and costs less time. The stage of NPC suspension culture can improve the purity and differentiation efficiency of neuron. Specially, we observed that functional synapse formed after day 23 by electrophysiological recording as well as a differential expression pattern emerged by day 7 by RNA-seq analysis, confirming by the expression of Wnt signaling pathway genes. Furthermore, neuron derived from patient iPSC can be used in drug screening and disease mechanism exploration, especially neurological diseases and neurodevelopment diseases, such as autism spectrum disorders, RETT syndrome, Alzheimer’s disease and schizophrenia. In 2013, Shcheglovitov19 proved the dysfunction of the ratio of excitation and inhibition neurons derived from PMDS patient iPSC and the synaptic deficits can be rescue by IGF1. What is more, translational research (such as cell transplantation) and genetic correction by CRISPR-Cas9 hold great promise for therapy of neurological diseases. Researchers had successfully corrected the defect gene form patient iPSC32, which can significantly promote the progress of efficient treatments for neurological diseases and neurodevelopment diseases.
