We intercrossed heterozygous mice to determine the biological function of Bptf during mouse development. With 210 mice genotyped for the BptfXG023 allele and 75 mice for the BptfΔExon2 allele, we did not find any surviving homozygous mice at weaning, indicating that Bptf is required for mouse development. An analysis of E6.5 to E18.5 progeny derived from heterozygous intercrosses confirmed that the homozygous mutant phenotype is embryonic-lethal between E7.5 to E8.5, with 100% penetrance (Table S1).
