Fibroblasts from a FAD patient carrying a PSEN1 A246E mutation (A15, female, 56 years old) and another FAD patient carrying a PSEN1 Ser169del mutation (A16, female, 45 years old) were obtained from the Xiangya Hospital with the approval of the Ethical Committee and informed consents of donors. Normal (N) fibroblasts from an unrelated subject were obtained from the Renji Hospital of Shanghai Jiao Tong University School of Medicine. Fibroblasts were cultured as previously described [49]. iPSC lines were established through infection of fibroblasts with retroviral vectors containing coding sequences of human OCT4, SOX2, KLF4 and C-MYC. Procedures for retroviral production, iPSC derivation and characterization were the same as previously described [50]. iPSCs were cultured on CF1 or ICR MEF feeders for expansion in the human ESC medium (for detail please see Supplementary Information).
