Genotyping was performed using a closed-tube fluorescent TaqMan 5’-nuclease allelic discrimination assay and MGB-probes and primers designed using Primer Express v3.0 software [Applied Biosystems Inc. (ABI) Foster City, CA]. Fluorescence plate reads and genotype calls were made using ABI 7900 Sequence Detection Systems. Two ng of genomic DNA were PCR amplified in 384-well plates using a 5-μl reaction volume. Repeat genotyping was completed for 20% of samples with a genotype discrepancy rate of 0.2%.
