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Chunk #12 — General Methods — Experimental Design

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Adolescent but not adult rats exhibit ethanol-mediated appetitive second-order conditioning.
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A 2 (age: adolescents or adults) × 2 (ethanol dosage: 0.5 or 2.0 g/kg) × 3 (conditioning procedure) factorial design defined the assessment of ethanol-mediated second-order conditioning. Subjects were exposed to the CS1 during an early (5-20 min; Early pairing, EP) or a later (30-45 min; Late pairing, LP) postadministration time, or the CS1 was explicitly unpaired with ethanol’s postabsorptive effects (Unpaired controls, UP). Groups had a minimum of 9 and a maximum of 12 animals.