described in the edgeR manual. DAVID (http://david.abcc.ncifcrf.gov/) was used to perform the gene functional annotation analysis. The categories of GO and KEGG pathways were chosen as background databases. All genes of Homo sapiens were used as background gene list. The RNA-seq data have been deposited in NCBI’s Gene Expression Omnibus under accession number GSE58933. The Prox1::eGFP-positive DG-like neurons showed gene expression similar to the whole differentiation culture (Extended Data Fig. 9).
