we performed a consensus analysis to examine possible involvement of these transcription factors in EtOH-induced molecular alterations in hESCs. We compared those genes altered by EtOH treatment against genes altered in hESCs treated with short hairpin RNA targeting OCT4 (shOCT4, obtained from publicly available data sets, GEO accession number: GSE34921). We found the significant modules of alterations specifically associated with the stemness transcription factor and EtOH treatment (Fig. S4). This further suggests that EtOH treatment in hESCs can lead to fundamental alterations in the regulatory network of transcription factors involved in stemness and lineage commitment (Fig. S4).
