the behavioral intoxication state of the rat as assessed with a six point scale: 0-normal rat (5 g/kg), 1-hypoactive (4 g/kg), 2-ataxia (3 g/kg), 3-delayed righting reflex and ataxia with dragging abdomen (2 g/kg), 4-loss of righting reflex (1 g/kg) and 5-loss of eye blink reflex (0 g/kg). Control rats received an isocaloric diet of Vanilla Ensure® and dextrose. To verify intoxication, blood ethanol concentrations (BECs) were determined from tail blood. On Day 2 of the binge, tail blood was extracted approximately 90 m after the afternoon dose of alcohol, centrifuged and stored at −20°C until analysis. BECs were determined from plasma on a GM7 Alcohol Analyser (Analox, Lunenburg, MA).
