Beyond such studies on protein replacement rates, there remains a pressing need to develop methodologies for studying the locus-specific dynamics of other aspects of chromatin structure. Is a highly H3K4-methylated nucleosome experiencing constant de- and remethylation, or is methylation stable once deposited? Are TADs unfolding and refolding over minute time-scales? These and many other questions await new measurement methods that explicitly address dynamic aspects of the epigenome.
