Across a larger panel of neural lineage markers (Table S4) in 23 hiPSC-astrocyte lines and their isogenic NPC lines, principal component analysis (PCA) revealed that the NPCs grouped together, while the astrocytes (both hiPSC-derived and primary human fetal astrocytes) were more dispersed (Figures 1F and S1N). Because it was apparent in our hiPSC-astrocytes as well as the primary human fetal astrocytes (obtained from different donors and brain regions), this variability in lineage marker expression may reflect inter-individual variability and/or differences in regional patterning (Figures 1G and S1O).
