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Chunk #12 — MATERIALS AND METHODS — ANIMAL EXPERIMENTATION — Western Blotting

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CYP2A6 is associated with obesity: studies in human samples and a high fat diet mouse model.
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The SDS-PAGE and chemiluminescence imaging was carried out as described before (ref. 9–12). β-actin was detected as a protein loading control. Anti-CYP2E1 and anti-CYP2A antibodies were gifts from Dr. Jerome Lasker (Hackensack Biomedical Research Institute, Hackensack, NJ, USA) and Dr. Risto Juvonen (Department of Pharmacology and Toxicology, University of Kuopio, Kuopio, Finland), respectively. Anti-ADRP was from Abcam (Cambridge, MA, USA).