In total, 513 DMRs (hypermethylated, 196; hypomethylated, 317) overlapped promoters or exons/introns of 492 annotated RefSeq genes. To gain insight into the broader functional roles of these DMR-associated genes, we assessed their potential enrichment in GO terms associated with biological processes, molecular functions, and cellular components; we combined genes associated with hyper- and hypomethylated DMRs to increase power in the analysis. The top 5 GO term enrichments and genes for each category are listed in Table 2 (see also Supplementary Table S2), highlighting functional involvement of DMR-associated genes in cell membrane function, animal behavior, synaptic organization, receptor activity, and also including proteins localized to cellular components of neurons and synapses. Genes contributing to GO enrichments were equally represented by hyper- and hypomethylated effects. The DMR state for each gene is indicated in Table 2. GO analysis results partitioned by hyper- and hypomethylated DMRs are also provided in Supplementary Tables S3 and S4.
