Male, Long Evans rats (Harlan, Indianapolis, IN) weighing 260–280 g upon arrival were individually housed in ventilated polycarbonate chambers (colony room temperature, 21 ± 1°C; 12-hour light/dark cycle with lights on at 7:00 a.m.). Rats had unrestricted access to standard rat chow and water throughout the study. All procedures were approved by the Gallo Center Institutional Animal Care and Use Committee.
