Chunk #16 — METHODS — UPR analysis

Source

TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.

Embedded

yes

Text

The XBP1 luciferase reporter assay was performed as for the NFκB/RE luciferase reporter assay but with the use of an XBP1 luciferase reporter construct and HT1080 cells (ATCC). ER stress-induced splicing of the 26-bp intron of human XBP1 was analyzed.