conditions due to aforementioned clustering of cells. To study outgrowth as an early process in the formation of neuronal networks, we imaged iPSC-derived neurons 50 hours after plating onto a substrate patterned with an array of flower-shaped features (Supplemental Movie 2). As shown in Figure 4A, neurons cultured on patterned substrates allowed for easy tracking of neurite outgrowth processes from one cell to another over time.
