Cell Culture Experiments, RNA extraction and qPCR: Lymphoblastoid cell lines (LCLs) from the AFR superpopulation were obtained from the Coriell Institute, NJ (Supplementary Table 13, N = 6 lines each for the homozygous major and homozygous minor allele). Cells were cultured in RPMI 1640 medium with GlutaMAX (Thermo Scientific, 61870-036) supplemented with 15% FBS (Thermo Scientific, 26140079) and 1X Antibiotic-Antimycotic (Thermo Scientific, 15240-062) at 37 C and 5% CO2 in a humidified incubator. For Dexamethasone (Dex) treatment, a final concentration of 100 nM Dex (Sigma–Aldrich) in 100% Ethanol was added to the medium for a total of 4 hr. All experiments were run in duplicates.
