As done for total ethanol-responsive gene sets, we investigated GO or pathway functional over-representation for the S-score networks. The vast majority of networks were over-represented for at least one gene family, protein domain/interaction, KEGG pathway or GO category, significant at a FDR level of 5% (Table S7). ErGeN1 was strikingly enriched for proteins with GTPase activity (p-value = 1.5E-07), including Rab3a, which mediates ataxic consequences of ethanol consumption and influences ethanol preference [59]. Both ErGeN1 and ErGeN3 were significantly enriched for genes encoding proteins that localize to the synapse (Table 4). In contrast, S-score networks 2 and 12 had a large over-representation of genes related to ribosome function and oxidative phosphorylation.
