Besides their cheap and easy handling, a lot is known about fibroblasts and they are well established in several fields of research. The first successful approach of reprogramming adult cells has been performed using mouse fibroblasts [1]. When this method was adapted to human cells, the first attempt was consequentially done with fibroblasts. As other groups started to reproduce this groundbreaking achievement, most protocols were also using fibroblasts before new techniques were investigated. Nevertheless, every new method can be improved, and since the starting material is a crucial variable in the whole reprogramming process, many groups have started to search for better alternatives as there are some disadvantages coming up with the use of fibroblasts. Fibroblasts are mesenchymal cells within the dermis layer of the skin and are responsible for producing precursor molecules, parts of the extracellular matrix. With respect to the generation of patient specific iPSCs, in most clinical approaches, it is nearly indispensable to harvest cells directly from the affected patient. Most commonly, normal human dermal fibroblasts are obtained either from adult skin biopsies or neonatal foreskin biopsies
