Chunk #33 — ONLINE METHODS — Live imaging

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Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture.

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For visualizing radial glia, hCSs were infected with a lentivirus expressing EGFP under the human GFAP promoter (Lenti-GFAP::EGFP). At day 52 of differentiation in vitro, hCSs were sliced and VZ-like regions were imaged at 37 °C with a Leica SP8 confocal microscope for up to three hours.