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Chunk #45 — Discussion — Methodological issue

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Topological organization of functional brain networks in healthy children: differences in relation to age, sex, and intelligence.
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The effects of age, sex, and IQ on the topological organization of brain networks in healthy children should be examined carefully and precisely. Significant interactions of age-by-sex, sex-by-IQ, or age-by-sex-by-IQ have been identified in previous studies of children on functional connectivity [97], [105], [106], anatomical connectivity [107], brain perfusion [23], and brain structure [50], [108], [109], [110], [111], [112]. Moreover, the significances in the regional node properties found in this study were at the level of p<0.05 without multiple comparisons correction; an even higher level of significance might be able to be achieved by including more subjects. Therefore, a large number of subjects will be useful for the investigation of the effects of age, sex, and IQ on brain network properties in future studies. Furthermore, sexually dimorphic trajectories in the brain structure [108], IQ-related trajectories of cortical development [110], and the established topological organization of brain anatomical networks in early brain development [55] have been confirmed by longitudinal designs. Therefore, although this study was a cross-sectional study, a longitudinal analysis would also be helpful to examine the developmental trajectory of