We used the PrimerPicker software (http://www.kbioscience.co.uk/primer-picker/) to design the assay and followed the protocol described in the KASPar SNP Genotyping System manual (http://www.kbioscience.co.uk), with detection on the ABI 7900 HT Fast Real-Time PCR system (Applied Biosystems, Foster City, California). Sixty-nine percent of samples were also genotyped using the Sequenom MassArray system. Samples with discrepant results (0.3%) were discarded.
