Biological triplicates of each condition were used to isolate RNA and were analyzed independently by RNA-seq using an Illumina HiSeq2500 instrument. The raw data were analyzed by the Tuxedo pipeline and aligned to the UCSC genome hg38build. The expression levels of over 24,331 genes were assessed and considered significant (16,137 genes) when expressed in all samples (Supplementary Table S2). The remaining 8194 genes were not detected in all samples and were eliminated from further analysis due to their near-threshold expression. A total of 4704 genes showed expression levels that differed significantly (FC ≥ ± 1.5 and q-value > 0.05) between nondifferentiated NPCs and their NCC progeny induced by differentiating factors (cAMP/BDNF/GDNF) (Supplementary Table S3). Three hundred and thirty-two genes were differentially expressed between NPCs and NPCs+ FGFR1(SP-/NLS(TK-) (Supplementary Table S4). Expression of 861 genes differed significantly in NCCs pre-transfected with FGFR1(SP-/NLS)(TK-), as compared to β-galactosidase (Supplementary Table S5). In NCCs, 440 genes were affected by transfection of active nFGFR1(SP-/NLS).
