Chunk #52 — Results and Discussion — AM3506 selectively elevated brain anandamide

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Convergent translational evidence of a role for anandamide in amygdala-mediated fear extinction, threat processing and stress-reactivity.

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there was a similarly potent and long-lasting inhibition of FAAH in cerebellar tissue extracts (Supplementary Figure S1). In the same samples, we also determined the activity of MAGL, by measuring the release of [3H]glycerol from 2-arachidonyl-[3H]glycerol. In contrast to FAAH inhibition, MAGL activity in the forebrain was unaltered by AM3506 treatment (n = 3–4) (Figure 1b). Thus, these data demonstrate that systemic administration of AM3506 produced a potent, lasting and preferential inhibition of FAAH in the brain.