The results of firing rate over the course of the entire 2CAP sessions for electrophysiology studies were recently described (Linsenbardt and Lapish, 2015). The primary goal herein was to evaluate cue-induced alterations in neural activity, which was not evaluated previously. Peristimulus time histograms (PSTHs) were created by aligning binned (100 ms) spike trains for each neuron to the onset of each trial. PSTHs were smoothed using a Gaussian function with a standard deviation of 300 ms, and softmax normalized to avoid being biased by high firing rate neurons by dividing the firing rate of each neuron by its maximum variance (Ames et al., 2014).
