We note also a recent cannabis GWAS publication37 implicated CHRNA2 (and no other locus) as associated with cannabis-use disorder. In our study, CHRM3 was demonstrated to be significantly co-expressed with CHRNA4 in the brain tissue THAL. CHRNA2 and CHRNA4 encode the nicotinic AChR subunit α-2 and α-4, respectively. Our result considered in that context provides additional weight to considering cholinergic signaling as a mechanism of risk for cannabis-use disorder and cannabis-induced harmful effects.
