To illustrate the several new features in g:Profiler we have used data published by Robert et al. (34). They analysed the total mRNA and the mRNA fraction associated with polysomes on human adipose tissue-derived stem cells (hASCs) at 24 h of osteogenesis induction. Differentially expressed genes (DEGs) were identified by paired comparisons between non-induced cells and induced cell conditions, and for polysomal and total RNA fractions. GO enrichment analysis of DEGs from both total and polysomal RNA fractions using previous g:Profiler version was part of their analysis pipeline. The enrichment results were then visualized using REVIGO (35). Most of the biological processes were enriched in both of the groups and related to response to external stimuli, cell communication and development.
