of 3 missed cleavages; precursor mass tolerance of 10 ppm; and a fragment mass tolerance of 0.5 Da, and MS3 FTMS reporter ion tolerance of 20 ppm. Values from both unique and razor peptides were used for quantification. In the consensus workflows, peptides were normalized by total peptide amount with no scaling. Data shown is for HAP/LAP abundance value ratios (AR). Sex/Line ratios are provided at https://github.com/dlhagger/AUD-Multi-Omics. Resulting grouped abundance values for each sample type, AR values; and respective p-values (t-test) from Proteome Discover™ were exported to Microsoft Excel.
