Chunk #41 — RESULTS — Transcription factors register the developmental history and contribute to the maintenance of PCP phenotypes — GABAergic neurons retain a transcription resume that registers their developmental history
Previous studies suggest that neuronal identities can be maintained by sustained expression of the same set of transcription factors that initiate terminal differentiation during development (Dalla Torre di Sanguinetto et al., 2008; Deneris and Hobert, 2014). In embryonic subpallium, transcriptional cascades orchestrate the specification and differentiation of major clades of GABAergic neurons (Kepecs and Fishell, 2014; Nord et al., 2015) (Figure 7A–B). We found that each PCP expresses ~350–400 TFs and over 300 TFs are expressed in an individual cell (Figure 7C). Among ~34 TF classes, basic-helix-loop-helix proteins, nuclear hormone receptors, POU-homeoboxes, and kruppel-like transcription factors are most differentially expressed among PCPs (Table S4, S3), and multiple TFs individually marks each PCP (Figure 7H, S7B).
