quantitative trait, approximately 8900, 4500, 3000 and 2300 independent individuals are required to detect of 0.1, 0.2, 0.3 and 0.4 with >80% power at a type-I error rate of 0.05, respectively. For two quantitative traits measured on the same sample, approximately 7000, 4700, 2500 and 1600 independent individuals are required to detect of 0.2, 0.4, 0.6 and 0.8 with >80% at a type-I error rate of 0.05, respectively.