Microglia and astrocyte clusters were further divided into four and two subclusters, respectively, by performing another iteration of FindNeighbors and FindClusters, using cells from the 143 samples. Subcluster-specific genes were determined by using the roc test within the FindMarkers function in Seurat (see https://satijalab.org/seurat/reference/findmarkers). The top 50 genes (based on myAUC statistic) were used as input into g:Profiler82 to determine enriched biological pathways specific to that subcluster. (Notably, g:Profiler uses their own method, called gSCS, for multiple testing correction in lieu of Benjamini–Hochberg (BH) method)
