Our goal was to model RNAseq data from mixed cultures of human neurons induced from iPSC lines co-cultured with mouse glia, since this is a system used in our research.19, 20, 33 Therefore, we obtained public FASTQ files of human control interneuron RNAseq data (GEO accession GSE118313, sample GSM3324649, 9,506,181 raw reads)34 and mouse astrocytes derived from dorsal root ganglia (GEO accession GSE133745, sample GSM3926526, 80,822,800 raw reads)35. Both datasets were paired-end with 75 nt/end.
