C. elegans were cultured at 20 °C on nematode growth media agar plates seeded with bacteria. Strains were provided by the Caenorhabditis Genetics Center (University of Minnesota). The strains used were SJ4100 (zcIs13[hsp-6::GFP]), SJ4005 (zcIs4[hsp-4::GFP] and dvIs70 [hsp-16.2p::GFP+rol-6(su1006)]. RNAi constructs were isolated from the RNAi feeding library (GeneService) and experiments were carried out using standard feeding methods. The identity of each RNAi clone was verified by sequencing. RNAi treatment was started at embryonic stage. GFP was monitored in day 1 adults. Worms were immobilized with 6 mM solution of tetramisole hydrochloride (Sigma) in M9 and imaged using Nikon DS-L2 fluorescent microscope.
