Another breeding strategy that is commonly used in inbred mouse studies is the backcross design. In this approach, the F1 offspring of two inbred progenitors are mated back to one of the progenitor inbred strains (i.e., are backcrossed), to generate a backcross generation. Compared with an F2 sample, the backcross offspring will not be as diverse with respect to their genotype or their phenotype.
