Many protocols for next-generation sequencing library preparation use PAGE, with excision and purification of the desired fragment range from the gel. However, gel-based size selection can result in melting of the two DNA strands, precluding its use with DS. In addition, the use of gels increases the introduction of DNA damage during UV transillumination. To mitigate these problems, we perform size selection with Ampure XP beads, which has the additional benefits of higher recovery and greater speed.
