The Fmn2−/− mice were generated using 129/SvEv (now strain 129S6/SvEvTac) derived TC-1 embryonic stem cells. Chimeric mice were backcrossed to 129/SvEv [70]. The Fmn2-null and littermate controls are therefore coisogenic. To validate the isogenicity of regions surrounding the targeted locus [112], we genotyped the Fmn2+/+, Fmn2+/−, and Fmn2−/− mice using ten microsatellite markers located on, and flanking Fmn2 (markers distributed from 172 Mb to 182 Mb). These markers are D1Mit455, D1Mit113, D1Mit456, D1Mit356, D1Mit206, D1Mit355, D1Mit150, D1Mit403, D1Mit315, and D1Mit426. With the exception of a marker at Fmn2 (D1Mit150), all alleles in null, heterozygote, and wildtype animals were identical.
