than that of a randomly chosen control29. An AUC of 0.5 indicates that a classifier does not provide any useful information in determining cases from controls (see supplemental information Section 3). We next divided PRSs at several thresholds (80th, 90th, and 95th percentiles) to examine whether there was a non-linear increase in risk of AUD (using symptom severity thresholds of AUD) across the PRS continuum. Finally, we compared mean values of other substance use outcomes for the top 5% in each PRS to those in the bottom 95%. We selected this threshold based in the increased prevalence of AUD in those in the top 5% of the PRS distributions (see Fig. S3). All code is available upon request from the corresponding author.
