We analyzed each putative eQTL hotspot using a penalized partial correlation graph.55 We highlight a network where a distant eQTL located on chr19 is also a local eQTL of MYOF1. Given the expression of SOX13, MYOF1 expression is independent of other distant eQTL genes (Figure 5d), suggesting the eQTL signals are mediated by SOX13. MYOF1 encodes unconventional myosins which bind to membranous compartments and serve in intracellular movements. SOX13 is a transcription factor that modulates the Wnt/TCF signaling pathway,56 and several other distant eQTL genes are involved in cellular signaling (e.g., TMEM134, RGS12, and SYT13). Although significant networks were found (Supplementary Figure 11), the relatively few genes influenced by hotspots or distant eQTLs suggests such networks do not play a predominant role in steady state transcription in peripheral blood.
