All cDNA clones printed on an array were sequenced. Briefly, plasmid DNA was extracted from the clones using a Templiphil™ DNA Sequencing Template Amplification kit (Amersham Biosciences, NJ) according to the manufacturer’s protocol. All clones were sequenced using standard dye primer chemistry by an ABI PRISM® 310 Genetic Analyzer (Applied Biosystems, CA). The resulting sequences from 12,000 cDNA clones (ca. 300–800 nucleotides in length) were searched against various public genomic databases to determine the function and category of each clone.
