Light/dark. The animals were acclimated to the darkened room for a minimum of 30 min. A lamp, with 15 W bulb was located directly above the light portion of the light/dark box which had total dimensions of 24.13 cm L × 45.72 cm W. To begin the 10-min test, animals were placed in the light half of the box. The guillotine door was then removed to allow the animals to freely move between the two halves of the box. The amount of time spent in the light vs. dark compartment was measured. Once an animal had been tested, it is placed in a holding cage until all animals from the home cage have been tested.
