Chunk #29 — Results — Low-density neuronal cultures show synaptic activity, produce action potentials and Ca2+ transients

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Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells.

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10% of the hESC-derived neurons, suggestive of intrinsic pacemaker activity in a small group of neurons. The network also responded to electrical field stimulation with increased activity/amplitude correlated to the number of pulses given (Fig 4K and S4E Fig). These results indicate that the hPSC-derived neurons trigger action potentials, establish active glutamatergic and GABAergic synapses and generate synaptic transmission in the neuronal network.