Blood samples were taken and DNA was extracted using the standard salting-out method. DNA was quantified using PicoGreen (Invitrogen, Carlsbad, CA). 700 ng DNA (45 μL) was ethanol-precipitated for a second time and re-suspended in 7 μl Tris-EDTA (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) as described (Levran et al., 2008). 5 μl (250-500 ng) were used for genotyping.
