For 15 probes that showed discrepant eQTL scores between the UC and UW analyses (i.e. BF>4 in one study and BF<4 in the other), we designed primers to capture the relevant expression array probe and amplified and Sanger-sequenced the resulting PCR products in each of the 60 UW liver samples and 35 CEU HapMap samples. SNPs were identified as previously described (http://pga.gs.washington.edu/) including both automated prediction and manual curation.
