The animal experimental procedures in this study were approved by the Institutional Animal Care and Use Committee at the Indiana University School of Medicine (Protocol Number 19017). Guidelines set forth by the National Institutes of Health (Maryland, USA) for ethical treatment and care for experimental animals were followed. Adult (postnatal day 60–75) male and female HAP and LAP mice bred and maintained at Indiana University-Purdue University Indianapolis (IUPUI) facilities were transferred to our laboratory prior to tissue collection. All mice were alcohol-naïve and group-housed at the time of tissue collection. The vivarium space was maintained at 20°C and 50% relative humidity with ad libitum access to food and water. These lines were described in the introduction and information related to their development may be found elsewhere (Matson and Grahame, 2013; Oberlin et al., 2011). For the current work, mice from the 66th generation of the second replicate were used. Replicate lines are used to ensure that the alcohol preference selection phenotype could be produced in multiple lines. Our choice to only test replicate two here was based on the similar
