these 101 genes. Finally, CHRM3 co-expressed genes that emerged in the top ten enriched gene categories of disease-associated genes were validated for co-expression with CHRM3 using ten different types of human healthy brain tissues (downloaded from Braineac database)17 and the homologous gene in mouse, Chrm3, using six types of cortical GABAergic neurons from mouse FCTX (gene expression omnibus accession number GSE92522)20. The ten brain tissues (total samples = 1340) are the same as these listed above. In addition, we considered six types of mouse single neurons (total samples = 584): martinotti cells, interneuron selective cells, cholecystokinin expressing (CCK)-basket cells, parvalbumin expressing (PV) basket cells, chandelier cells upper layer (CHC1) and deep layer (CHC2) of mouse brain, and long projecting cells. All of the cells were isolated from mouse FCTX. The correlation between CHRM3 and its co-expressed genes emerged in the enrichment of disease-associated genes was analyzed separately among ten brain tissues for Pearson’s correlation by using MATLAB (Statistics Toolbox Release 2015b, The MathWorks, Inc., Natick, Massachusetts, USA). The same correlation analysis was performed for the mouse homologous Chrm3 among six types of single neurons. The adjusted P-value for significance was 0.05/(10*24) = 2 × 10−4 for the co-expression analysis with
