Trace files (.ab1) were analyzed using the ESME version 3.2.1 software from Epigenomics AG (Berlin, Germany) (Lewin et al, 2004). The ESME software was run on a virtual machine (VMware player running on Windows XP) running Red Hat Enterprise Linux 4. The percent methylation calls by the ESME were reviewed by two independent researchers who visually inspected all the methylation calls using the associated electropherograms generated by the ESME software.
