pooled from four animals were loaded into a single well of a Chromium chip G (10x Genomics). 3’ gene expression libraries were generated using Chromium Next GEM Single Cell 3’ Kit 3.1 with 3’ Feature Barcode Kit and dual indexing (10x Genomics protocol CG000316 Rev D). Libraries were sequenced as PE150 by Illumina sequencing to approximately 65% saturation. Reads were mapped to the mm10 mouse genome (GENCODE vM23/Ensembl 98) using the 10x Genomics Cell Ranger pipeline (6.0.0) with default settings.
