A 1,536 tag SNP addiction array has been developed within the Laboratory of Neurogenetics, NIAAA, allowing for the analysis of 130 candidate genes for the addictions and for 186 ancestry informative markers [56]. Genotypes for 8 SNPs across the corticotropin releasing hormone binding protein (CRH-BP) gene were available from the addiction array for 310 Plains Indians with EEG data (Table 4) and for 186–188 of the U.S. Caucasians (Table 5). As described above, we calculated that the average percentage of genetic identity shared between any two individuals through common descent for all possible pairs (related and unrelated) was 0.3%, which is less than the degree of relationship between third cousins. Therefore ANOVA was performed to test the association between CRH-BP genotypes and EEG power. The significant p values were confirmed by 10,000 simulations using SIMPED [57]. With this program genotypes can be generated conditional on the user-specified frequencies using the gene-drop method within pedigrees of virtually any size and complexity. Since the four significant SNPs were in allelic identity, association analysis (t-test between the two homozygous genotypes) was computed for
