To make these differential activity motif results more robust, we also utilized a pseudobulk approach: averaging per-cell motif scores for all cells within a sample of a given cell type, taking the log, and then using an ANOVA—with sex, age, and ethnic origin as covariates—to test for differences between those with and without AUD. All samples used for differential accessibility testing (see “ATAC pseudobulk samples creation”) were used for this analysis.
